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tom20 cib stop  (Addgene inc)


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    Addgene inc tom20 cib stop
    Tom20 Cib Stop, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tom20 cib stop/product/Addgene inc
    Average 92 stars, based on 6 article reviews
    tom20 cib stop - by Bioz Stars, 2026-03
    92/100 stars

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    tom20 cib stop  (Addgene inc)
    92
    Addgene inc tom20 cib stop
    Tom20 Cib Stop, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tom20 cib stop/product/Addgene inc
    Average 92 stars, based on 1 article reviews
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    tom20-cib-stop  (Addgene inc)
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    An optogenetic targeting of CLIC4 to the mitochondria delays cell division. (A) Schematic of Mitotrap used in this experiment. In all cases cells expressing endogenously tagged GFP–CLIC4 were co-transfected with Cry2-GFP-VHH and <t>CIB-Tom20</t> plasmids and pulsed with a 488 nm laser to re-target GFP–CLIC4 at the mitochondria. (B) Interphase cell exposed to 488 nm to activate Mitotrap. Mitochondria are labeled in red and endogenous GFP–CLIC4 in green. (C,D) Still images from time-lapse microscopy where Mitotrap was activated. Arrows point to blebs or cytokinesis failure induced by GFP–CLIC4 Mitotrap. Scale bars: 100 μm. (E) Quantification of time required for cells to complete mitotic cell division in control and Mitotrapped cells. Data shown are the means and standard deviations.
    Tom20 Cib Stop, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tom20-cib-stop/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    tom20-cib-stop - by Bioz Stars, 2026-03
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    An optogenetic targeting of CLIC4 to the mitochondria delays cell division. (A) Schematic of Mitotrap used in this experiment. In all cases cells expressing endogenously tagged GFP–CLIC4 were co-transfected with Cry2-GFP-VHH and CIB-Tom20 plasmids and pulsed with a 488 nm laser to re-target GFP–CLIC4 at the mitochondria. (B) Interphase cell exposed to 488 nm to activate Mitotrap. Mitochondria are labeled in red and endogenous GFP–CLIC4 in green. (C,D) Still images from time-lapse microscopy where Mitotrap was activated. Arrows point to blebs or cytokinesis failure induced by GFP–CLIC4 Mitotrap. Scale bars: 100 μm. (E) Quantification of time required for cells to complete mitotic cell division in control and Mitotrapped cells. Data shown are the means and standard deviations.

    Journal: Journal of Cell Science

    Article Title: CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division

    doi: 10.1242/jcs.241117

    Figure Lengend Snippet: An optogenetic targeting of CLIC4 to the mitochondria delays cell division. (A) Schematic of Mitotrap used in this experiment. In all cases cells expressing endogenously tagged GFP–CLIC4 were co-transfected with Cry2-GFP-VHH and CIB-Tom20 plasmids and pulsed with a 488 nm laser to re-target GFP–CLIC4 at the mitochondria. (B) Interphase cell exposed to 488 nm to activate Mitotrap. Mitochondria are labeled in red and endogenous GFP–CLIC4 in green. (C,D) Still images from time-lapse microscopy where Mitotrap was activated. Arrows point to blebs or cytokinesis failure induced by GFP–CLIC4 Mitotrap. Scale bars: 100 μm. (E) Quantification of time required for cells to complete mitotic cell division in control and Mitotrapped cells. Data shown are the means and standard deviations.

    Article Snippet: The following plasmids were used: pCAG:IRES-CLIC4 (a gift from the Sung Laboratory, Cornell University, Ithaca, NY), pEGFP-N3:CLIC4, RhoA FRET biosensor (a gift from Gary Bokoch, Scripps Research Institute, San Diego, CA), pGEX:CLIC4, pCMV-SNAP-CRY2-VHH(GFP) (58370; Addgene, Watertown, MA), Tom20-CIB-stop (117243; Addgene), Cry2PHR-mCH-RhoA (42959; Addgene) and pGEX6P1-GFP-Nanobody (61838; Addgene).

    Techniques: Expressing, Transfection, Labeling, Time-lapse Microscopy